Flaminal^® Hydro combines non-cytotoxicity with antimicrobial activity

Dave Van den Plas, Wounds-UK 2005

A study to determine the cytotoxicity and antibacterial activity of Flaminal^® Hydro was performed.  Cytotoxicity was studied by incubating fibroblast (3T3) and keratinocyte cells (HaCat) for 24 hours with wound products dissolved in growth medium.  Cell viability was determined by performing an MTT assay and cell division determined by cell uptake of a thymidine analogue.  Antibacterial activity was assessed by adding microbial suspensions to Flaminal^® Hydro. At different time points, samples were taken and plated on agar plates. After overnight incubation, surviving bacterial and fungal cells were assessed by determining the number of colony forming units (CFU).

	Flaminal® Hydro had no influence on the metabolic activity and proliferation of keratinocytes or fibroblasts, in vitro.
	Flaminal® Hydro demonstrated a high degree of activity against Gram positive bacteria (S aureus), Gram negative bacteria (E coli) and yeasts (C albicans), in vivo.

The results show that Flaminal^® Hydro combines effective control of microbial burden with non-cytotoxicity towards two important cell types in wound healing.  This in vitro data might explain the improved overall healing witnessed in a clinical study with patients suffering from ulcers.

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