Flaminal^® Hydro combines non-cytotoxicity
with antimicrobial activity

Van den Plas D, Wounds-UK 2005

A study to determine the cytotoxicity and antibacterial activity of Flaminal^® Hydro was
performed. Cytotoxicity was studied by incubating fibroblast (3T3) and keratinocyte cells
(HaCat) for 24 hours with wound products dissolved in growth medium. Cell viability was
determined by performing an MTT assay and cell division determined by cell uptake of a
thymidine analogue. Antibacterial activity was assessed by adding microbial suspensions
to Flaminal^® Hydro. At different time points, samples were taken and plated on agar plates.
After overnight incubation, surviving bacterial and fungal cells were assessed by determining
the number of colony forming units (CFU).

Flaminal^® Hydro had no influence on the metabolic activity and proliferation of
     keratinocytes or fibroblasts, in vitro

Flaminal^® Hydro demonstrated a high degree of activity against Gram positive bacteria
     (S aureus), Gram negative bacteria (E coli) and yeasts (C albicans), in vivo

The results show that Flaminal^® Hydro combines effective control of microbial burden
with non-cytotoxicity towards two important cell types in wound healing. This in vitro data
might explain the improved overall healing witnessed in a clinical study with patients suffering
from ulcers.

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